A novel approach to measure mitochondrial respiration in frozen biological samples.
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ABSTRACT:
SUBMITTER: Acin-Perez R
PROVIDER: S-EPMC7327496 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
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A novel approach to measure mitochondrial respiration in frozen biological samples.
Acin-Perez Rebeca R Benador Ilan Y IY Petcherski Anton A Veliova Michaela M Benavides Gloria A GA Lagarrigue Sylviane S Caudal Arianne A Vergnes Laurent L Murphy Anne N AN Karamanlidis Georgios G Tian Rong R Reue Karen K Wanagat Jonathan J Sacks Harold H Amati Francesca F Darley-Usmar Victor M VM Liesa Marc M Divakaruni Ajit S AS Stiles Linsey L Shirihai Orian S OS
The EMBO journal 20200520 13
Respirometry is the gold standard measurement of mitochondrial oxidative function, as it reflects the activity of the electron transport chain complexes working together. However, the requirement for freshly isolated mitochondria hinders the feasibility of respirometry in multi-site clinical studies and retrospective studies. Here, we describe a novel respirometry approach suited for frozen samples by restoring electron transfer components lost during freeze/thaw and correcting for variable perm ...[more]
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