Sphingolipid distribution at mitochondria-associated membranes (MAMs) upon induction of apoptosis.

ABSTRACT: The levels and composition of sphingolipids and related metabolites are altered in aging and in common disorders such as diabetes and cancers, as well as in neurodegenerative, cardiovascular, and respiratory diseases. Changes in sphingolipids have been implicated as being an essential step in mitochondria-driven cell death. However, little is known about the precise sphingolipid composition and modulation in mitochondria or related organelles. Here, we used LC-MS/MS to analyze the presence of key components of the ceramide metabolic pathway in vivo and in vitro in purified ER, mitochondria-associated membranes (MAMs), and mitochondria. Specifically, we analyzed the sphingolipids in the three pathways that generate ceramide: sphinganine in the de novo ceramide pathway, SM in the breakdown p

SUBMITTER: Mignard V 

PROVIDER: S-EPMC7328052 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature