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Peptidomic data of egg white gastrointestinal digests prepared using the Infogest Harmonized Protocol.

ABSTRACT: These data are related to the research article entitled "Induction of CCK and GLP-1 release in enteroendocrine cells by egg white peptides generated during gastrointestinal digestion". In this article, the peptide and free amino acid profile of egg white gastrointestinal in vitro digests is shown. Egg white proteins were digested following the INFOGEST gastrointestinal digestion protocol. Different time points of gastric and intestinal digestion were characterized regarding protein, peptide and amino acid content. Protein degradation was followed by SDS-PAGE where some electrophoretic bands were identified by MALDI-TOF/TOF after tryptic digestion. Moreover, the molecular weight distribution of egg white peptides found at different times of gastrointestinal digestion was performed using MALDI-TOF. Peptides identified from the most abundant egg white proteins by tandem mass spectrometry were represented using a peptide profile tool and raw data are given in table format. These results reveal the protein regions resistant to digestion and illustrate the free amino acid profile of egg white protein at the end of the digestion process. These data can be used for nutritional purposes and to identify allergen epitopes or bioactive sequences.

SUBMITTER: Santos-Hernandez M

PROVIDER: S-EPMC7334575 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Peptidomic data of egg white gastrointestinal digests prepared using the Infogest Harmonized Protocol.

Santos-Hernández Marta M Miralles Beatriz B Amigo Lourdes L Recio Isidra I

Data in brief 20200626

These data are related to the research article entitled "Induction of CCK and GLP-1 release in enteroendocrine cells by egg white peptides generated during gastrointestinal digestion". In this article, the peptide and free amino acid profile of egg white gastrointestinal <i>in vitro</i> digests is shown. Egg white proteins were digested following the INFOGEST gastrointestinal digestion protocol. Different time points of gastric and intestinal digestion were characterized regarding protein, pepti ...[more]

PMID: 32642529

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Project description:BackgroundThe anonymization of Common Data Model (CDM)-converted EHR data is essential to ensure the data privacy in the use of harmonized health care data. However, applying data anonymization techniques can significantly affect many properties of the resulting data sets and thus biases research results. Few studies have reviewed these applications with a reflection of approaches to manage data utility and quality concerns in the context of CDM-formatted health care data.ObjectiveOur intended scoping review aims to identify and describe (1) how formal anonymization methods are carried out with CDM-converted health care data, (2) how data quality and utility concerns are considered, and (3) how the various CDMs differ in terms of their suitability for recording anonymized data.MethodsThe planned scoping review is based on the framework of Arksey and O'Malley. By using this, only articles published in English will be included. The retrieval of literature items should be based on a literature search string combining keywords related to data anonymization, CDM standards, and data quality assessment. The proposed literature search query should be validated by a librarian, accompanied by manual searches to include further informal sources. Eligible articles will first undergo a deduplication step, followed by the screening of titles. Second, a full-text reading will allow the 2 reviewers involved to reach the final decision about article selection, while a domain expert will support the resolution of citation selection conflicts. Additionally, key information will be extracted, categorized, summarized, and analyzed by using a proposed template into an iterative process. Tabular and graphical analyses should be addressed in alignment with the PRISMA-ScR (Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews) checklist. We also performed some tentative searches on Web of Science for estimating the feasibility of reaching eligible articles.ResultsTentative searches on Web of Science resulted in 507 nonduplicated matches, suggesting the availability of (potential) relevant articles. Further analysis and selection steps will allow us to derive a final literature set. Furthermore, the completion of this scoping review study is expected by the end of the fourth quarter of 2023.ConclusionsOutlining the approaches of applying formal anonymization methods on CDM-formatted health care data while taking into account data quality and utility concerns should provide useful insights to understand the existing approaches and future research direction based on identified gaps. This protocol describes a schedule to perform a scoping review, which should support the conduction of follow-up investigations.International registered report identifier (irrid)PRR1-10.2196/46471.

Project description:Salmonella enterica serovar Enteritidis is noted for its ability to survive the harsh antibacterial activity of egg white which is presumed to explain its occurrence as the major food-borne pathogen associated with the consumption of eggs and egg products. Liquid egg white is a major ingredient for the food industry but, because of its thermal fragility, pasteurization is performed at the modest temperature of 57°C (for 2-6 min). Unfortunately, such treatment does not lead to sufficient reduction in S. Enteritidis contamination, which is a clear health concern when the product is consumed without cooking. However, egg white is able to limit S. Enteritidis growth due to its alkaline pH, iron deficiency and multiple antimicrobial proteins. This anti-Salmonella activity of egg white is temperature dependent and becomes bactericidal once the incubation temperature exceeds 42°C. This property is exploited in the highly promising pasteurization treatment (42-45°C for 1-5 days) which achieves complete killing of S. Enteritidis. However, the precise mechanism and the role of the egg-white proteins are not fully understood. Here, the impact of exposure of S. Enteritidis to egg white-based media, with or without egg-white proteins (>10 kDa), under bactericidal conditions (45°C) was explored by measuring survival and global expression. Surprisingly, the bactericidal activity of egg white at 45°C was only slightly affected by egg-white proteins indicating that they play a minor role in the bactericidal activity observed. Moreover, egg-white proteins had minimal impact on the global-gene-expression response to egg white such that very similar, major regulatory responses (20% genes affected) were observed both with and without egg-white proteins following incubation for 45 min at 45°C. Egg-white proteins caused a significant change in expression for just 64 genes, including the psp and lysozyme-inhibitor responses genes which is suggestive of an early membrane perturbation effect. Such damage was supported by disruption of the proton motive force by egg-white proteins. In summary, the results suggest that low-mass components of egg white are largely responsible for the bactericidal activity of egg white at 45°C.

Dataset Information

Peptidomic data of egg white gastrointestinal digests prepared using the Infogest Harmonized Protocol.

Publications

Peptidomic data of egg white gastrointestinal digests prepared using the Infogest Harmonized Protocol.

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