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JYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths.

ABSTRACT: Femtosecond lasers at fixed wavelengths above 1,000?nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000?nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.

SUBMITTER: Mohr MA

PROVIDER: S-EPMC7335340 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths.

Mohr Manuel Alexander MA Bushey Daniel D Aggarwal Abhi A Marvin Jonathan S JS Kim Jeong Jun JJ Marquez Emiliano Jimenez EJ Liang Yajie Y Patel Ronak R Macklin John J JJ Lee Chi-Yu CY Tsang Arthur A Tsegaye Getahun G Ahrens Allison M AM Chen Jerry L JL Kim Douglas S DS Wong Allan M AM Looger Loren L LL Schreiter Eric R ER Podgorski Kaspar K

Nature methods 20200525 7

Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluoresce ...[more]

PMID: 32451475

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