Effect of Divalent Cations on the Structure and Mechanics of Vimentin Intermediate Filaments.

ABSTRACT: Divalent cations behave as effective cross-linkers of intermediate filaments (IFs) such as vimentin IF (VIF). These interactions have been mostly attributed to their multivalency. However, ion-protein interactions often depend on the ion species, and these effects have not been widely studied in IFs. Here, we investigate the effects of two biologically important divalent cations, Zn²⁺ and Ca²⁺, on VIF network structure and mechanics in vitro. We find that the network structure is unperturbed at micromolar Zn²⁺ concentrations, but strong bundle formation is observed at a concentration of 100 μM. Microrheological measurements show that network stiffness increases with cation concentration. However, bundling of filaments softens the network. This trend also ho

SUBMITTER: Wu H 

PROVIDER: S-EPMC7335910 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature