Ontology highlight
ABSTRACT:
SUBMITTER: Fracchiolla D
PROVIDER: S-EPMC7337497 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Fracchiolla Dorotea D Chang Chunmei C Hurley James H JH Martens Sascha S
The Journal of cell biology 20200701 7
Autophagy degrades cytoplasmic cargo by its delivery to lysosomes within double membrane autophagosomes. Synthesis of the phosphoinositide PI(3)P by the autophagic class III phosphatidylinositol-3 kinase complex I (PI3KC3-C1) and conjugation of ATG8/LC3 proteins to phagophore membranes by the ATG12-ATG5-ATG16L1 (E3) complex are two critical steps in autophagosome biogenesis, connected by WIPI2. Here, we present a complete reconstitution of these events. On giant unilamellar vesicles (GUVs), LC3 ...[more]