Ontology highlight
ABSTRACT:
SUBMITTER: Schneider G
PROVIDER: S-EPMC7337972 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Schneider Gerd G Guttmann Peter P Heim Stefan S Rehbein Stefan S Mueller Florian F Nagashima Kunio K Heymann J Bernard JB Müller Waltraud G WG McNally James G JG
Nature methods 20101114 12
We developed an X-ray microscope using partially coherent object illumination instead of previously used quasi-incoherent illumination. The design permitted the incorporation of a cryogenic tilt stage, enabling tomography of frozen-hydrated, intact adherent cells. We obtained three-dimensional reconstructions of mouse adenocarcinoma cells at ∼36-nm (Rayleigh) and ∼70-nm (Fourier ring correlation) resolution, which allowed us to visualize the double nuclear membrane, nuclear pores, nuclear membra ...[more]