Three-dimensional cellular ultrastructure resolved by X-ray microscopy.

ABSTRACT: We developed an X-ray microscope using partially coherent object illumination instead of previously used quasi-incoherent illumination. The design permitted the incorporation of a cryogenic tilt stage, enabling tomography of frozen-hydrated, intact adherent cells. We obtained three-dimensional reconstructions of mouse adenocarcinoma cells at ?36-nm (Rayleigh) and ?70-nm (Fourier ring correlation) resolution, which allowed us to visualize the double nuclear membrane, nuclear pores, nuclear membrane channels, mitochondrial cristae and lysosomal inclusions.

SUBMITTER: Schneider G 

PROVIDER: S-EPMC7337972 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature