Ontology highlight
ABSTRACT:
SUBMITTER: Ding W
PROVIDER: S-EPMC7338305 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Ding Wentao W Zhang Yang Y Shi Shuobo S
Frontiers in bioengineering and biotechnology 20200630
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools with high efficiency, accuracy and flexibility, and has revolutionized traditional methods for applications in microbial biotechnology. Here, key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA. Following an overview of various CRISPR/Cas tools ...[more]