Dataset Information

Initial experience with [18F]DPA-714 TSPO-PET to image inflammation in primary angiitis of the central nervous system.

ABSTRACT: PURPOSE:Primary angiitis of the central nervous system (PACNS) is a heterogeneous, rare, and poorly understood inflammatory disease. We aimed at non-invasive imaging of activated microglia/macrophages in patients with PACNS by PET-MRI targeting the translocator protein (TSPO) with [18F]DPA-714 to potentially assist differential diagnosis, therapy monitoring, and biopsy planning. METHODS:In total, nine patients with ischemic stroke and diagnosed or suspected PACNS underwent [18F]DPA-714-PET-MRI. Dynamic PET scanning was performed for 60 min after injection of 233?±?19 MBq [18F]DPA-714, and MRI was simultaneously acquired. RESULTS:In two PACNS patients, [18F]DPA-714 uptake patterns exceeded MRI correlates of infarction, whereas uptake was confined to the infarct in four patients where initial suspicion of PACNS could not be confirmed. About three patients with PACNS or cerebral predominant lymphocytic vasculitis showed no or only faintly increased uptake. Short-term [18F]DPA-714-PET follow-up in a patient with PACNS showed reduced lesional [18F]DPA-714 uptake after anti-inflammatory treatment. Biopsy in the same patient pinpointed the source of tracer uptake to TSPO-expressing immune cells. CONCLUSIONS:[18F]DPA-714-PET imaging may facilitate the diagnosis and treatment monitoring of PACNS. Further studies are needed to fully understand the potential of TSPO-PET in deciphering the heterogeneity of the disease.

SUBMITTER: Backhaus P

PROVIDER: S-EPMC7338821 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Initial experience with [<sup>18</sup>F]DPA-714 TSPO-PET to image inflammation in primary angiitis of the central nervous system.

Backhaus Philipp P Roll Wolfgang W Beuker Carolin C Zinnhardt Bastian B Seifert Robert R Wenning Christian C Eisenblätter Michel M Thomas Christian C Schmidt-Pogoda Antje A Strunk Daniel D Wagner Stefan S Faust Andreas A Tüttelmann Frank F Röpke Albrecht A Jacobs Andreas H AH Stummer Walter W Wiendl Heinz H Meuth Sven G SG Schäfers Michael M Grauer Oliver O Minnerup Jens J

European journal of nuclear medicine and molecular imaging 20200120 9

<h4>Purpose</h4>Primary angiitis of the central nervous system (PACNS) is a heterogeneous, rare, and poorly understood inflammatory disease. We aimed at non-invasive imaging of activated microglia/macrophages in patients with PACNS by PET-MRI targeting the translocator protein (TSPO) with [<sup>18</sup>F]DPA-714 to potentially assist differential diagnosis, therapy monitoring, and biopsy planning.<h4>Methods</h4>In total, nine patients with ischemic stroke and diagnosed or suspected PACNS underw ...[more]

PMID: 31960097

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Project description:BACKGROUND:Cerebral stroke is a severe and frequent condition that requires rapid and reliable diagnosis. If administered shortly after the first symptoms manifest themselves, IV thrombolysis has been shown to increase the functional prognosis by restoring brain reperfusion. However, a better understanding of the pathophysiology of stroke should help to identify potential new therapeutic targets. Stroke is known to induce an inflammatory brain reaction that involves overexpression of the 18-kDa translocator protein (TSPO) in glial cells and infiltrated leukocytes, which can be visualised by positron emission tomography (PET). We aimed to evaluate post-stroke neuroinflammation using the PET TSPO radioligand (18)?F-DPA-714. METHODS:Nine patients underwent (18)?F-DPA-714 PET and magnetic resonance imaging (MRI) between 8 and 18 days after the ictus. Co-registration of MRI and PET images was used to define three volumes of interest (VOIs): core infarction, contralateral region, and cerebellum ipsilateral to the stroke lesion. Time activity curves were obtained from each VOI, and ratios of mean and maximum activities between the VOIs were calculated. RESULTS:We observed an increased uptake of (18)?F-DPA-714 co-localised with the infarct tissue and extension beyond the region corresponding to the damage in the blood brain barrier. No correlation was identified between (18)?F-DPA-714 uptake and infarct volume. (18)?F-DPA-714 uptake in ischemic lesion (mainly associated with TSPO expression in the infarct area and in the surrounding neighbourhood) slowly decreased from 10 min pi to the end of the PET acquisition, remaining higher than that in both contralateral region and ipsilateral cerebellum. CONCLUSION:Our results show that (18)?F-DPA-714 uptake after acute ischemia is mainly associated with TSPO expression in the infarct area and in the surrounding neighbourhood. We also demonstrated that the kinetics of (18)?F-DPA-714 differs in injured tissue compared to normal tissue. Therefore, (18)?F-DPA-714 may be useful in assessing the extent of neuroinflammation associated with acute stroke and could also help to predict clinical outcomes and functional recovery, as well as to assess therapeutic strategies, such as the use of neuroprotective/anti-inflammatory drugs.

Dataset Information

Initial experience with [18F]DPA-714 TSPO-PET to image inflammation in primary angiitis of the central nervous system.

Publications

Initial experience with [<sup>18</sup>F]DPA-714 TSPO-PET to image inflammation in primary angiitis of the central nervous system.

Similar Datasets

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