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Enzymatic reaction-based nanopore detection of zinc ions.

ABSTRACT: We report a label-free nanopore sensor for the detection of Zn2+ ions. By taking advantage of the cleavage of a substrate peptide by zinc-dependent enzymes, nanomolar concentrations of Zn2+ ions could be detected within minutes. Furthermore, structurally similar transition metals such as Ni2+, Co2+, Hg2+, Cu2+, and Cd2+ did not interfere with their detection. The enzymatic reaction-based nanopore sensing strategy developed in this work may find potential applications in environmental monitoring and medical diagnosis.

SUBMITTER: Roozbahani GM

PROVIDER: S-EPMC7341991 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Enzymatic reaction-based nanopore detection of zinc ions.

Roozbahani Golbarg Mohammadi GM Zhang Youwen Y Chen Xiaohan X Soflaee Mona Hoseini MH Guan Xiyun X

The Analyst 20191201 24

We report a label-free nanopore sensor for the detection of Zn2+ ions. By taking advantage of the cleavage of a substrate peptide by zinc-dependent enzymes, nanomolar concentrations of Zn2+ ions could be detected within minutes. Furthermore, structurally similar transition metals such as Ni2+, Co2+, Hg2+, Cu2+, and Cd2+ did not interfere with their detection. The enzymatic reaction-based nanopore sensing strategy developed in this work may find potential applications in environmental monitoring ...[more]

PMID: 31691699

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