Ontology highlight
ABSTRACT:
SUBMITTER: Loussert-Fonta C
PROVIDER: S-EPMC7347930 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Communications biology 20200709 1
Correlative light and electron microscopy allows localization of specific molecules at the ultrastructural level in biological tissue but does not provide information about metabolic turnover or the distribution of labile molecules, such as micronutrients. We present a method to directly correlate (immuno)fluorescent microscopy, (immuno)TEM imaging and NanoSIMS isotopic mapping of the same tissue section, with nanometer-scale spatial precision. The process involves chemical fixation of the tissu ...[more]