Ontology highlight
ABSTRACT:
SUBMITTER: Guo RR
PROVIDER: S-EPMC7348039 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Frontiers in bioengineering and biotechnology 20200703
Peptide-<i>N</i> <sup>4</sup>-(<i>N</i>-acetyl-β-glucosaminyl) asparagine amidases (PNGases, <i>N</i>-glycanases, EC 3.5.1.52) are indispensable tools in releasing <i>N</i>-glycans from glycoproteins. So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties. Herein, a panel of 13 novel PNGase H<sup>+</sup> candidates (the suffix H<sup>+</sup> refers to the acidic pH optimum of these acidobacterial PNGases) was tested ...[more]