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Role for Cell-Surface Collagen of Streptococcus pyogenes in Infections.

ABSTRACT: Group A Streptococcus (GAS) displays cell-surface proteins that resemble human collagen. We find that a fluorophore-labeled collagen mimetic peptide (CMP) labels GAS cells but not Escherichia coli or Bacillus subtilis cells, which lack such proteins. The CMP likely engages in a heterotrimeric helix with endogenous collagen, as the nonnatural d enantiomer of the CMP does not label GAS cells. To identify a molecular target, we used reverse genetics to "knock-in" the GAS genes that encode two proteins with collagen-like domains, Scl1 and Scl2, into B. subtilis. The fluorescent CMP labels the cells of these B. subtilis strains. Moreover, these strains bind tightly to a surface of mammalian collagen. These data are consistent with streptococcal collagen forming triple helices with damaged collagen in a wound bed and thus have implications for microbial virulence.

SUBMITTER: Ellison AJ

PROVIDER: S-EPMC7354224 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Role for Cell-Surface Collagen of Streptococcus pyogenes in Infections.

Ellison Aubrey J AJ Dempwolff Felix F Kearns Daniel B DB Raines Ronald T RT

ACS infectious diseases 20200603 7

Group A Streptococcus (GAS) displays cell-surface proteins that resemble human collagen. We find that a fluorophore-labeled collagen mimetic peptide (CMP) labels GAS cells but not Escherichia coli or Bacillus subtilis cells, which lack such proteins. The CMP likely engages in a heterotrimeric helix with endogenous collagen, as the nonnatural d enantiomer of the CMP does not label GAS cells. To identify a molecular target, we used reverse genetics to "knock-in" the GAS genes ...[more]

PMID: 32413256

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