Ontology highlight
ABSTRACT:
SUBMITTER: Ellison AJ
PROVIDER: S-EPMC7354224 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Ellison Aubrey J AJ Dempwolff Felix F Kearns Daniel B DB Raines Ronald T RT
ACS infectious diseases 20200603 7
Group A <i>Streptococcus</i> (GAS) displays cell-surface proteins that resemble human collagen. We find that a fluorophore-labeled collagen mimetic peptide (CMP) labels GAS cells but not <i>Escherichia coli</i> or <i>Bacillus subtilis</i> cells, which lack such proteins. The CMP likely engages in a heterotrimeric helix with endogenous collagen, as the nonnatural d enantiomer of the CMP does not label GAS cells. To identify a molecular target, we used reverse genetics to "knock-in" the GAS genes ...[more]