Ontology highlight
ABSTRACT:
SUBMITTER: Mejia YX
PROVIDER: S-EPMC4311812 | biostudies-literature | 2015 Jan
REPOSITORIES: biostudies-literature
Mejia Yara X YX Nudler Evgeny E Bustamante Carlos C
Proceedings of the National Academy of Sciences of the United States of America 20141231 3
Two components of the RNA polymerase (RNAP) catalytic center, the bridge helix and the trigger loop (TL), have been linked with changes in elongation rate and pausing. Here, single molecule experiments with the WT and two TL-tip mutants of the Escherichia coli enzyme reveal that tip mutations modulate RNAP's pause-free velocity, identifying TL conformational changes as one of two rate-determining steps in elongation. Consistent with this observation, we find a direct correlation between helix pr ...[more]