Automated high content image analysis of dendritic arborization in primary mouse hippocampal and rat cortical neurons in culture.

ABSTRACT:

Background

Primary neuronal cell cultures are useful for studying mechanisms that influence dendritic morphology during normal development and in response to various stressors. However, analyzing dendritic morphology is challenging, particularly in cultures with high cell density, and manual methods of selecting neurons and tracing dendritic arbors can introduce significant bias, and are labor-intensive. To overcome these challenges, semi-automated and automated methods are being developed, with most software solutions requiring computer-assisted dendrite tracing with subsequent quantification of various parameters of dendritic morphology, such as Sholl analysis. However fully automated approaches for classic Sholl analysis of dendritic complexity are not currently available.

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SUBMITTER: Schmuck MR 

PROVIDER: S-EPMC7357201 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature