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Norepinephrine transporter-derived homing peptides enable rapid endocytosis of drug delivery nanovehicles into neuroblastoma cells.

ABSTRACT: BACKGROUND:Currently, the diagnosis and treatment of neuroblastomas-the most frequent solid tumors in children-exploit the norepinephrine transporter (hNET) via radiolabeled norepinephrine analogs. We aim to develop a nanomedicine-based strategy towards precision therapy by targeting hNET cell-surface protein with hNET-derived homing peptides. RESULTS:The peptides (seq. GASNGINAYL and SLWERLAYGI) were shown to bind high-resolution homology models of hNET in silico. In particular, one unique binding site has marked the sequence and structural similarities of both peptides, while most of the contribution to the interaction was attributed to the electrostatic energy of Asn and Arg (

SUBMITTER: Haddad Y

PROVIDER: S-EPMC7359476 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Norepinephrine transporter-derived homing peptides enable rapid endocytosis of drug delivery nanovehicles into neuroblastoma cells.

Haddad Yazan Y Charousova Marketa M Zivotska Hana H Splichal Zbynek Z Merlos Rodrigo Miguel Angel MA Michalkova Hana H Krizkova Sona S Tesarova Barbora B Richtera Lukas L Vitek Petr P Stokowa-Soltys Kamila K Hynek David D Milosavljevic Vedran V Rex Simona S Heger Zbynek Z

Journal of nanobiotechnology 20200713 1

<h4>Background</h4>Currently, the diagnosis and treatment of neuroblastomas-the most frequent solid tumors in children-exploit the norepinephrine transporter (hNET) via radiolabeled norepinephrine analogs. We aim to develop a nanomedicine-based strategy towards precision therapy by targeting hNET cell-surface protein with hNET-derived homing peptides.<h4>Results</h4>The peptides (seq. GASNGINAYL and SLWERLAYGI) were shown to bind high-resolution homology models of hNET in silico. In particular, ...[more]

PMID: 32660596

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Imaging the norepinephrine transporter in neuroblastoma: a comparison of [18F]-MFBG and 123I-MIBG.

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Inorganic Kernel-Reconstituted Lipoprotein Biomimetic Nanovehicles Enable Efficient Targeting "Trojan Horse" Delivery of STAT3-Decoy Oligonucleotide for Overcoming TRAIL Resistance.

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