Unknown

Dataset Information

0

Upregulation of microRNA-451 attenuates myocardial I/R injury by suppressing HMGB1.


ABSTRACT: Both MicroRNAs and HMGB1 took part in pathological process of myocardial I/R injury though several signaling pathways. We hypothesized that mircoRNA451 (miR-451), a group of small non-coding RNAs, could improve this injury by inhibiting HMGB1. Male SD rats were randomly distributed into 5 groups and subjected to I/R process. After 24 hours of reperfusion injury, the serum content of CK and LDH, the content of MDA in tissue and activity of SOD were detected; The infarcted areas were defined by TTC staining and Evans Blue; TUNEL staining and cleaved-Caspase 3 were used to test apoptosis; HMGB1 was detected by real-time fluorescence quantitative PCR and Western Blotting. Compared with the I/R and I/R+Ad-GFP group, upregulation of miR-451 could reduce the infarcted areas, cardiomyocytes apoptosis index, expression of cleaved-caspase 3 and content of CK and LDH significantly(P<0.05); Meanwhile, upregulation of miR-451 could also obviously inhibit HMGB1, the increase of MDA and the decrease of SOD (P<0.05). So this study revealed that upregulation of miR-451 could prevent myocardial I/R injury by suppressing HMGB1.

SUBMITTER: Cao J 

PROVIDER: S-EPMC7367451 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

altmetric image

Publications

Upregulation of microRNA-451 attenuates myocardial I/R injury by suppressing HMGB1.

Cao Jianlei J   Da Yurong Y   Li Hang H   Peng Yuanyuan Y   Hu Xiaorong X  

PloS one 20200717 7


Both MicroRNAs and HMGB1 took part in pathological process of myocardial I/R injury though several signaling pathways. We hypothesized that mircoRNA451 (miR-451), a group of small non-coding RNAs, could improve this injury by inhibiting HMGB1. Male SD rats were randomly distributed into 5 groups and subjected to I/R process. After 24 hours of reperfusion injury, the serum content of CK and LDH, the content of MDA in tissue and activity of SOD were detected; The infarcted areas were defined by TT  ...[more]

Similar Datasets

| S-EPMC8428118 | biostudies-literature
| S-EPMC8631436 | biostudies-literature
| S-EPMC7874400 | biostudies-literature
| S-EPMC3480558 | biostudies-literature
| S-EPMC4090021 | biostudies-literature
| S-EPMC6748144 | biostudies-literature
| S-EPMC7485697 | biostudies-literature
| S-EPMC7260042 | biostudies-literature
| S-EPMC5746084 | biostudies-other
| S-EPMC7522513 | biostudies-literature