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Advances and challenges in genetic technologies to produce single-sex litters.

ABSTRACT: There is currently a requirement for single-sex litters for many applications, including agriculture, pest control, and reducing animal culling in line with the 3Rs principles: Reduction, Replacement, and Refinement. The advent of CRISPR/Cas9 genome editing presents a new opportunity with which to potentially generate all-female or all-male litters. We review some of the historical nongenetic strategies employed to generate single-sex litters and investigate how genetic and genome editing techniques are currently being used to produce all-male or all-female progeny. Lastly, we speculate on future technologies for generating single-sex litters and the possible associated challenges.

SUBMITTER: Douglas C

PROVIDER: S-EPMC7377362 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Advances and challenges in genetic technologies to produce single-sex litters.

Douglas Charlotte C Turner James M A JMA

PLoS genetics 20200723 7

There is currently a requirement for single-sex litters for many applications, including agriculture, pest control, and reducing animal culling in line with the 3Rs principles: Reduction, Replacement, and Refinement. The advent of CRISPR/Cas9 genome editing presents a new opportunity with which to potentially generate all-female or all-male litters. We review some of the historical nongenetic strategies employed to generate single-sex litters and investigate how genetic and genome editing techni ...[more]

PMID: 32701961

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