Ontology highlight
ABSTRACT:
SUBMITTER: Nguyen HT
PROVIDER: S-EPMC7378944 | biostudies-literature | 2020 Jan-Dec
REPOSITORIES: biostudies-literature
Nguyen Hiep Thi HT Nghia Nguyen Trong NT Lien Nghiem Thi Ha NTH Dang-Nguyen Thanh Quang TQ Men Nguyen Thi NT Viet Linh Nguyen N Xuan Nguyen Bui B Noguchi Junko J Kaneko Hiroyuki H Kikuchi Kazuhiro K
Animal science journal = Nihon chikusan Gakkaiho 20200101 1
We examined the allelic expression and positioning of two pluripotency-associated genes, OCT4 and SOX2, and two housekeeping genes, ACTB and TUBA, in 4- and 8-cell porcine embryos utilizing RNA and DNA fluorescence in situ hybridization (FISH) in single blastomeres. The proportion of blastomeres expressing SOX2 bi-allelically increased from 45% at the 4-cell stage to 60% at the 8-cell stage. Moreover, in 8-cell embryos, SOX2 was expressed bi-allelically in significantly more blastomeres than was ...[more]