Ontology highlight
ABSTRACT:
SUBMITTER: Bhargava HK
PROVIDER: S-EPMC7382272 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Bhargava Hersh K HK Tabata Keisuke K Byck Jordan M JM Hamasaki Maho M Farrell Daniel P DP Anishchenko Ivan I DiMaio Frank F Im Young Jun YJ Yoshimori Tamotsu T Hurley James H JH
Proceedings of the National Academy of Sciences of the United States of America 20200706 29
Rubicon is a potent negative regulator of autophagy and a potential target for autophagy-inducing therapeutics. Rubicon-mediated inhibition of autophagy requires the interaction of the C-terminal Rubicon homology (RH) domain of Rubicon with Rab7-GTP. Here we report the 2.8-Å crystal structure of the Rubicon RH domain in complex with Rab7-GTP. Our structure reveals a fold for the RH domain built around four zinc clusters. The switch regions of Rab7 insert into pockets on the surface of the RH dom ...[more]