Ontology highlight
ABSTRACT:
SUBMITTER: Kara E
PROVIDER: S-EPMC7383712 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Kara Emrah E Nielsen Nis Valentin NV Eggertsdottir Bergrun B Thiede Bernd B Kanse Sandip M SM Løset Geir Åge GÅ
Chembiochem : a European journal of chemical biology 20200414 13
We describe a novel, easy and efficient combinatorial phage display peptide substrate-mining method to map the substrate specificity of proteases. The peptide library is displayed on the pVII capsid of the M13 bacteriophage, which renders pIII necessary for infectivity and efficient retrieval, in an unmodified state. As capture module, the 3XFLAG was chosen due to its very high binding efficiency to anti-FLAG mAbs and its independency of any post-translational modification. This library was test ...[more]