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Three-dimensional super-resolution fluorescence imaging of DNA.

ABSTRACT: Recent advances in fluorescence super-resolution microscopy are providing important insights into details of cellular structures. To acquire three dimensional (3D) super-resolution images of DNA, we combined binding activated localization microscopy (BALM) using fluorescent double-stranded DNA intercalators and optical astigmatism. We quantitatively establish the advantage of bis- over mono-intercalators before demonstrating the approach by visualizing single DNA molecules stretched between microspheres at various heights. Finally, the approach is applied to the more complex environment of intact and damaged metaphase chromosomes, unravelling their structural features.

SUBMITTER: Yardimci S

PROVIDER: S-EPMC7385144 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Three-dimensional super-resolution fluorescence imaging of DNA.

Yardimci Sevim S Burnham Daniel R DR Terry Samantha Y A SYA Yardimci Hasan H

Scientific reports 20200727 1

Recent advances in fluorescence super-resolution microscopy are providing important insights into details of cellular structures. To acquire three dimensional (3D) super-resolution images of DNA, we combined binding activated localization microscopy (BALM) using fluorescent double-stranded DNA intercalators and optical astigmatism. We quantitatively establish the advantage of bis- over mono-intercalators before demonstrating the approach by visualizing single DNA molecules stretched between micr ...[more]

PMID: 32719468

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