Histone modification dynamics as revealed by multicolor immunofluorescence-based single-cell analysis.

ABSTRACT: Post-translational modifications on histones can be stable epigenetic marks or transient signals that can occur in response to internal and external stimuli. Levels of histone modifications fluctuate during the cell cycle and vary among different cell types. Here, we describe a simple system to monitor the levels of multiple histone modifications in single cells by multicolor immunofluorescence using directly labeled modification-specific antibodies. We analyzed histone H3 and H4 modifications during the cell cycle. Levels of active marks, such as acetylation and H3K4 methylation, were increased during the S phase, in association with chromatin duplication. By contrast, levels of some repressive modifications gradually increased during G2 and the next G1 phases. We applied this method to v

SUBMITTER: Hayashi-Takanaka Y 

PROVIDER: S-EPMC7390643 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature