Light microscopy of proteins in their ultrastructural context.
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ABSTRACT:
SUBMITTER: M'Saad O
PROVIDER: S-EPMC7395138 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Publications
Light microscopy of proteins in their ultrastructural context.
M'Saad Ons O Bewersdorf Joerg J
Nature communications 20200731 1
Resolving the distribution of specific proteins at the nanoscale in the ultrastructural context of the cell is a major challenge in fluorescence microscopy. We report the discovery of a new principle for an optical contrast equivalent to electron microscopy (EM) which reveals the ultrastructural context of the cells with a conventional confocal microscope. By decrowding the intracellular space through 13 to 21-fold physical expansion while simultaneously retaining the proteins, bulk (pan) labeli ...[more]
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