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Correlative microscopy for phylogenetic and ultrastructural characterization of microbial communities.

ABSTRACT: Transmission electron microscopy (TEM) can provide ultrastructural information for cells in microbial community samples and phylogenetic information can be recovered via molecular surveys. Here we report an approach to link these data sets by coupling fluorescence in situ hybridization (FISH) with either conventional biological or cryogenic TEM. The method could fundamentally improve our understanding of the organization and functioning of microbial communities in natural systems.

SUBMITTER: Knierim B

PROVIDER: S-EPMC4444221 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

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Correlative microscopy for phylogenetic and ultrastructural characterization of microbial communities.

Knierim Bernhard B Luef Birgit B Wilmes Paul P Webb Richard I RI Auer Manfred M Comolli Luis R LR Banfield Jillian F JF

Environmental microbiology reports 20110822 1

Transmission electron microscopy (TEM) can provide ultrastructural information for cells in microbial community samples and phylogenetic information can be recovered via molecular surveys. Here we report an approach to link these data sets by coupling fluorescence in situ hybridization (FISH) with either conventional biological or cryogenic TEM. The method could fundamentally improve our understanding of the organization and functioning of microbial communities in natural systems. ...[more]

PMID: 23757227

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Correlative microscopy for phylogenetic and ultrastructural characterization of microbial communities.

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Correlative microscopy for phylogenetic and ultrastructural characterization of microbial communities.

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