Transcriptome profiling reveals signaling conditions dictating human spermatogonia fate in vitro.

ABSTRACT: Spermatogonial stem cells (SSCs) are essential for the generation of sperm and have potential therapeutic value for treating male infertility, which afflicts >100 million men world-wide. While much has been learned about rodent SSCs, human SSCs remain poorly understood. Here, we molecularly characterize human SSCs and define conditions favoring their culture. To achieve this, we first identified a cell-surface protein, PLPPR3, that allowed purification of human primitive undifferentiated spermatogonia (uSPG) highly enriched for SSCs. Comparative RNA-sequencing analysis of these enriched SSCs with differentiating SPG (KIT⁺ cells) revealed the full complement of genes that shift expression during this developmental transition, including genes encoding key components in the TGF-β,

SUBMITTER: Tan K 

PROVIDER: S-EPMC7395452 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature