Whole Genome Sequencing of Peruvian Klebsiella pneumoniae Identifies Novel Plasmid Vectors Bearing Carbapenem Resistance Gene NDM-1.

ABSTRACT: Background:Klebsiella pneumoniae is a bacterial pathogen with increasing rates of resistance to carbapenem antibiotics, but the population structure and genetic drivers of carbapenem-resistant K pneumoniae (CRKP) remain underexplored in developing countries. Carbapenem-resistant K pneumoniae were recently introduced into Peru but have grown rapidly in prevalence, enabling study of this pathogen as it expands into an unaffected environment. Methods:In this study, using whole genome sequencing, we show that 3 distinct lineages encompass almost all CRKP identified in the hospital where it was first reported in Peru. Results:The most prevalent lineage, ST348, has not been described outside of Europe, raising concern for global dissemination. We identified metallo- ? -lactamase NDM-1 as the primary carbapenem resistance effector, which was harbored on a novel vector resulting from recombination between 2 different plasmids, pKP1-NDM-1 and pMS7884A. Conclusions:This study is the first of its kind performed in Peru, and it furthers our understanding of the landscape of CRKP infections in Latin America.

SUBMITTER: Roach D 

PROVIDER: S-EPMC7395672 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature