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Rapid and Sensitive Method for Extraction of Plicosepalus acacia with Determination of Its Main Polyphenolic Compounds Using Validated HPLC.

ABSTRACT: Matrix solid phase dispersion (MSPD) trailed by HPLC is a quick and fruitful technique utilized for fortitude of flavonoids such as Catechin, Kaempferol, Quercetin, and Rutin existing in P. acacia. The trial parameters that influenced the extraction potential (comprising the mass ratio of sample to the dispersant, nature of dispersant, and the nature of elution solvent and its volume) were examined and optimized. These MSPD optimized parameters regulated are as follows: 8?mL of methanol was utilized as elution solvent, silica gel/sample mass ration was selected to be 2?:?1, and dispersing sorbent was silica gel. The technique retrievals were regulated to be "from 96.87 to 100.54%" and the RSDs from 1.24 to 4.45%. The product of extract obtained by MSPD method was larger than that of other methods, i.e., sonication extraction or traditional reflux with lessened necessities on time, sample, and solvent.

SUBMITTER: AlZain MN

PROVIDER: S-EPMC7396025 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Rapid and Sensitive Method for Extraction of Plicosepalus acacia with Determination of Its Main Polyphenolic Compounds Using Validated HPLC.

AlZain Mashail N MN Herqash Rashed N RN Almoqbil Abdulaziz N AN Mohammed Almarfadi Omer O Ibrahim Mansour N MN Ullah Riaz R Noman Omar M OM

Journal of analytical methods in chemistry 20200724

Matrix solid phase dispersion (MSPD) trailed by HPLC is a quick and fruitful technique utilized for fortitude of flavonoids such as Catechin, Kaempferol, Quercetin, and Rutin existing in P. acacia. The trial parameters that influenced the extraction potential (comprising the mass ratio of sample to the dispersant, nature of dispersant, and the nature of elution solvent and its volume) were examined and optimized. These MSPD optimized parameters regulated are as follows: 8 mL of met ...[more]

PMID: 32774985

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Project description:BACKGROUND:Acacia hydaspica belongs to family leguminosae possess antioxidant, anti-inflammatory and anticancer activities. During our search for antioxidant compounds from A. hydaspica, we carried out bioassay guided fractionation and obtained antioxidant compounds with free radical scavenging activity. MATERIALS AND METHODS:The polyphenol compounds in the plant extract of A. hydaspica were isolated by combination of different chromatographic techniques involving vacuum liquid chromatography and medium pressure liquid chromatography. The structural heterogeneity of isolated compounds was characterized by high pressure liquid chromatography, MS-ESI and NMR spectroscopic analyses. The antioxidant potential of isolated compounds has been investigated by 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide scavenging potential, hydroxyl radical scavenging potential, ferric reducing/antioxidant power (FRAP) model systems and total antioxidant capacity measurement. RESULTS:The isolated compounds show the predominance of signals representative of 7-O-galloyl catechins, catechins and methyl gallate. Flash chromatographic separation gives 750 mg of 7-O galloyl catechin, 400 mg of catechin and 150 mg of methyl gallate from 4 g loaded fraction on ISCO. Results revealed that C1 was the most potent compound against DPPH (EC50 1.60 ± 0.035 µM), nitric oxide radical (EC50 6 ± 0.346 µM), showed highest antioxidant index (1.710 ± 0.04) and FRAP [649.5 ± 1.5 µM Fe(II)/g] potency at 12.5 µM dose compared to C2, C3 and standard reference, whereas C3 showed lower EC50 values (4.33 ± 0.618 µM) in OH radical scavenging assay. CONCLUSION:Present research reports for the first time the antioxidant activity of polyphenolic compounds of A. hydaspica. Result showed good resolution and separation from other constituents of extract and method was found to be simple and precise. The isolation of catechin from this new species could provide a varied opportunity to obtain large quantities of catechin and catechin isomers beside from green tea. Free radical scavenging properties of isolated catechin isomers from A. hydaspica merit further investigations for consumption of this plant in oxidative stress related disorders.

Project description:This study presents a comparison between two mistletoe plants-P. acacia and P. curviflorus-regarding their total phenolic contents and antioxidant and anticancer activities. P. curviflorus exhibited a higher total phenolics content (340.62 ± 19.46 mg GAE/g extract), and demonstrated higher DPPH free radical scavenging activity (IC50 = 48.28 ± 3.41µg/mL), stronger reducing power (1.43 ± 0.54 mMol Fe+2/g) for ferric ions, and a greater total antioxidant capacity (41.89 ± 3.15 mg GAE/g) compared to P. acacia. The cytotoxic effects of P. acacia and P. curviflorus methanol extracts were examined on lung (A549), prostate (PC-3), ovarian (A2780) and breast (MDA-MB-231) cancer cells. The highest anticancer potential for the two extracts was observed on PC-3 prostate cancer cells, where P. curviflorus exhibited more pronounced antiproliferative activity (IC50 = 25.83 μg/mL) than P. acacia (IC50 = 34.12 μg/mL). In addition, both of the tested extracts arrested the cell cycle at the Pre-G1 and G1 phases, and induced apoptosis. However, P. curviflorus extract possessed the highest apoptotic effect, mediated by the upregulation of p53, Bax, and caspase-3, 8 and 9, and the downregulation of Bcl-2 expression. In the pursuit to link the chemical diversity of P. curviflorus with the exhibited bioactivities, its metabolomic profiling was achieved by the LC-ESI-TOF-MS/MS technique. This permitted the tentative identification of several phenolics-chiefly flavonoid derivatives, beside some triterpenes and sterols-in the P. curviflorus extract. Furthermore, all of the metabolites in P. curviflorus and P. acacia were inspected for their binding modes towards both CDK-2 and EGFR proteins using molecular docking studies in an attempt to understand the superiority of P. curviflorus over P. acacia regarding their antiproliferative effect on PC-3 cancer cells. Docking studies supported our experimental results; with all of this taken together, P. curviflorus could be regarded as a potential prospect for the development of chemotherapeutics for prostate cancer.

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Dataset Information

Rapid and Sensitive Method for Extraction of Plicosepalus acacia with Determination of Its Main Polyphenolic Compounds Using Validated HPLC.

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Rapid and Sensitive Method for Extraction of <i>Plicosepalus acacia</i> with Determination of Its Main Polyphenolic Compounds Using Validated HPLC.

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