Toxicological study of aqueous-methanol solvent fraction of methanol extract of Dacryodes edulis leaves.

ABSTRACT: Dacryodes edulis (G. Don) H.J. Lam) is the most popular species under the genus Dacryodes. It is well known for its nutritional and ethno-medicinal uses in South-eastern and South-western Nigeria. This study was aimed to evaluate the toxicity of the aqueous-methanol fraction of crude methanol extract of Dacryodes edulis leaves (AMDE). The test rats were randomized to groups of single oral treatment of AMDE (10-5000?mg/kgbw) for the acute toxicity study. They were monitored for obvious signs of behavioural change and mortality. For the subacute toxicity study, the rats were randomized to three daily treatment groups (of 200, 400 and 600?mg/kgbw of AMDE) for 28 days. The fourth group (control) received 2.5 %v/v DMSO. At the end of the experiment, blood samples were collected for hematology and clinical chemistry evaluation. The histopathology of the livers and kidneys were assessed using the excised organs. The cytotoxicity and genotoxicity of AMDE were also evaluated using Allium cepa model. The result showed that acute administration of AMDE, up to a dose of 5000?mg/kgbw did not result in mortality of the test rats. The observed median lethal dose (LD50) was greater than 5000?mg/kgbw. The subacute oral administration of AMDE for 28 days showed no significant (p?>?0.05) effect on liver function, kidney function indices, organ - body weight ratio, but significantly (p?

SUBMITTER: Ononamadu CJ 

PROVIDER: S-EPMC7398976 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature