Molecular basis of the lipid-induced MucA-MucB dissociation in Pseudomonas aeruginosa.
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ABSTRACT:
SUBMITTER: Li T
PROVIDER: S-EPMC7400510 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
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Molecular basis of the lipid-induced MucA-MucB dissociation in Pseudomonas aeruginosa.
Li Tao T He Lihui L Li Changcheng C Kang Mei M Song Yingjie Y Zhu Yibo Y Shen Yalin Y Zhao Ninglin N Zhao Chang C Yang Jing J Huang Qin Q Mou Xingyu X Tong Aiping A Yang Jinliang J Wang Zhenling Z Ji Chengjie C Li Hong H Tang Hong H Bao Rui R
Communications biology 20200803 1
MucA and MucB are critical negative modulators of sigma factor AlgU and regulate the mucoid conversion of Pseudomonas aeruginosa. Previous studies have revealed that lipid signals antagonize MucA-MucB binding. Here we report the crystal structure of MucB in complex with the periplasmic domain of MucA and polyethylene glycol (PEG), which unveiled an intermediate state preceding the MucA-MucB dissociation. Based on the biochemical experiments, the aliphatic side chain with a polar group was found ...[more]
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