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MiR?802 inhibits the epithelial?mesenchymal transition, migration and invasion of cervical cancer by regulating BTF3.

ABSTRACT: MicroRNA (miR)-802 has been discovered to be involved in the occurrence and development of numerous types of tumor; however, studies into the role of miR?802 in cervical cancer are limited. Therefore, the present study aimed to investigate the regulatory effects of miR?802 in cervical cancer cells. miR?802 expression levels in cervical cancer tissue and cells were analyzed using reverse transcription?quantitative (RT?q)PCR, a dual?reporter luciferase activity assay was used to identify the direct target gene of miR?802, and RT?qPCR and western blotting were performed to determine the relationship between miR?802 and basic transcription factor 3 (BTF3). Cell viability, and migration and invasion were analyzed using Cell Counting Kit?8 and Transwell assays, respectively. Finally, the expression levels of metastasis?associated proteins, N?cadherin and E?cadherin, were determined using RT?qPCR and western blotting. Decreased expression levels of miR?802 were found in cervical cancer tissues and cells, and the overexpression of miR?802 inhibited cell viability, migration and invasion. Moreover, miR?802 was discovered to directly target BTF3 to inhibit its expression. Notably, the overexpression miR?802 markedly reversed the promotive effect of BTF3 on cell viability, in addition to the migratory and invasive abilities of the cells. Simultaneously, the overexpression of miR?802 significantly suppressed epithelial?mesenchymal transition, and the expression levels of matrix metallopeptidase (MMP)2 and MMP9 in cells through regulating BTF3. In conclusion, the present study revealed that miR?802 may suppress cervical cancer progression by decreasing BTF3 expression levels, indicating that it may represent a potential therapeutic target for the treatment and prognosis of patients with cervical cancer.

SUBMITTER: Wu X

PROVIDER: S-EPMC7411396 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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miR‑802 inhibits the epithelial‑mesenchymal transition, migration and invasion of cervical cancer by regulating BTF3.

Wu Xiuhui X Liu Leng L Zhang Hongxia H

Molecular medicine reports 20200623 3

MicroRNA (miR)-802 has been discovered to be involved in the occurrence and development of numerous types of tumor; however, studies into the role of miR‑802 in cervical cancer are limited. Therefore, the present study aimed to investigate the regulatory effects of miR‑802 in cervical cancer cells. miR‑802 expression levels in cervical cancer tissue and cells were analyzed using reverse transcription‑quantitative (RT‑q)PCR, a dual‑reporter luciferase activity assay was used to identify the direc ...[more]

PMID: 32582971

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Project description:BackgroundThe present study is aimed at exploring the specific expression of miR-193a-3p and the mechanism underlying miR-193a-3p-mediated mesenchymal transition (MT), invasion, and migration in glioma.MethodsThe gene expression profile datasets of GSE39486 and GSE25676 were downloaded from the National Center for Biotechnology (NCBI). Data regarding the expression of miR-193a-3p and survival curves were derived from Chinese Glioma Genome Atlas (CGGA). Online websites including miRWalk, DIANA, and starbase were employed to predict the target genes for miR-193a-3p. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed by the Omicsbean online software. Module analysis of the protein-protein interaction (PPI) networks was performed by the plug-in Molecular Complex Detection (MCODE), and the degrees of genes were calculated by CytoHubba plug-in of Cytoscape. Survival curves were based on the Gene Expression Profile Interaction Analysis (GEPIA). Transwell, wound healing, and Western blot experiments were performed to investigate the effects of miR-193a-3p and beta-transducin repeat containing E3 ubiquitin protein ligase (BTRC) on the invasion, migration, and MT of glioma.ResultsmiR-193a-3p was highly expressed in glioma tissues and significantly correlated with poor survival in patients with glioma. The target genes for miR-193a-3p were involved in many cancer-related signaling pathways. The PPI showed 11 genes with both high degrees and MCODE scores in the network. Survival analysis demonstrated that the expression of BTRC was significantly correlated with the prognosis of patients with glioma. The results from the transwell, wound healing, and Western blot analyses suggested that miR-193a-3p promoted the invasion, migration, and MT of glioma cells, which could be reversed by BTRC.ConclusionsmiR-193a-3p was upregulated in patients with glioma and could affect the invasion, migration, and MT of glioma by regulating BTRC.

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MiR?802 inhibits the epithelial?mesenchymal transition, migration and invasion of cervical cancer by regulating BTF3.

Publications

miR‑802 inhibits the epithelial‑mesenchymal transition, migration and invasion of cervical cancer by regulating BTF3.

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