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Amarogentin from Gentiana rigescens Franch Exhibits Antiaging and Neuroprotective Effects through Antioxidative Stress.

ABSTRACT: In the present study, the replicative lifespan assay of yeast was used to guide the isolation of antiaging substance from Gentiana rigescens Franch, a traditional Chinese medicine. A compound with antiaging effect was isolated, and the chemical structure of this molecule as amarogentin was identified by spectral analysis and compared with the reported data. It significantly extended the replicative lifespan of K6001 yeast at doses of 1, 3, and 10??M. Furthermore, amarogentin improved the survival rate of yeast under oxidative stress by increasing the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx), and these enzymes' gene expression. In addition, this compound did not extend the replicative lifespan of sod1, sod2, uth1, and skn7 mutants with K6001 background. These results suggested that amarogentin exhibited antiaging effect on yeast via increase of SOD2, CAT, GPx gene expression, enzyme activity, and antioxidative stress. Moreover, we evaluated antioxidant activity of this natural products using PC12 cell system, a useful model for studying the nervous system at the cellular level. Amarogentin significantly improved the survival rate of PC12 cells under H2O2-induced oxidative stress and increased the activities of SOD and SOD2, and gene expression of SOD2, CAT, GPx, Nrf2, and Bcl-x1. Meanwhile, the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) of PC12 cells were significantly reduced after treatment of the amarogentin. These results indicated that antioxidative stress play an important role for antiaging and neuroprotection of amarogentin. Interestingly, amarogentin exhibited neuritogenic activity in PC12 cells. Therefore, the natural products, amarogentin from G. rigescens with antioxidant activity could be a good candidate molecule to develop drug for treating neurodegenerative diseases.

SUBMITTER: Disasa D

PROVIDER: S-EPMC7421772 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Amarogentin from Gentiana rigescens Franch Exhibits Antiaging and Neuroprotective Effects through Antioxidative Stress.

Disasa Dejene D Cheng Lihong L Manzoor Majid M Liu Qian Q Wang Ying Y Xiang Lan L Qi Jianhua J

Oxidative medicine and cellular longevity 20200801

In the present study, the replicative lifespan assay of yeast was used to guide the isolation of antiaging substance from Gentiana rigescens Franch, a traditional Chinese medicine. A compound with antiaging effect was isolated, and the chemical structure of this molecule as amarogentin was identified by spectral analysis and compared with the reported data. It significantly extended the replicative lifespan of K6001 yeast at doses of 1, 3, and 10 μM. Furthermore, amarogentin improv ...[more]

PMID: 32831994

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Project description:Gentiana rigescens Franch. ex Hemsl. is an important medicinal plant in China and the over exploitation of wild resources has affected its quality and clinical efficacy. The accumulation of plant secondary metabolites is not only determined by their genetic characteristics but also influenced by environmental factors. At present, many studies on evaluating the environmental conditions of its planting area are still in the qualitative stage. Therefore, it is necessary to establish a systematic evaluation method to deeply analyze the impact of environmental factors on the quality of medicinal materials and quickly verify the geographical origin. In this study, the contents of five iridoids (loganic acid, swertiamarin, sweroside, gentiopicroside and 6'-O-β-D-glucopyranosylgentiopicroside) of G. rigescens from 45 different origins (including 441 individuals) of Yunnan Province in China were analyzed by high performance liquid chromatography. Analytical procedures of one-way analysis of variance, correlation analysis, principal components analysis, and hierarchical cluster analysis were employed to interpret the correlation of iridoid content and environmental factors. Fourier transform infrared spectroscopy (FT-IR) combined with two multivariate analysis methods (partial least squares discriminant analysis; support vector machines, SVM) was used to discriminate four major producing areas (158 individuals). The combination of SVM with grid search algorithm achieved an accuracy of 100% in the test set. One-way analysis of variance showed that the contents of five iridoids in root tissues of G. rigescens varied significantly among different origins, which was also verified by the chemometrics analysis results of hierarchical cluster analysis. The results of correlation analysis indicated that the high value of altitude and precipitation were unfavorable for the accumulation of these five iridoids. A correlation between increase of temperature and iridoid accumulation was observed. This study provided a certain theoretical basis for the resource protection and development of G. rigescens based on the correlation analysis between the ecological environment factors and quality.

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Amarogentin from Gentiana rigescens Franch Exhibits Antiaging and Neuroprotective Effects through Antioxidative Stress.

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Amarogentin from <i>Gentiana rigescens</i> Franch Exhibits Antiaging and Neuroprotective Effects through Antioxidative Stress.

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