Influence of 16S rRNA target region on the outcome of microbiome studies in soil and saliva samples.
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ABSTRACT:
SUBMITTER: Soriano-Lerma A
PROVIDER: S-EPMC7423937 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Publications
Influence of 16S rRNA target region on the outcome of microbiome studies in soil and saliva samples.
Soriano-Lerma Ana A Pérez-Carrasco Virginia V Sánchez-Marañón Manuel M Ortiz-González Matilde M Sánchez-Martín Victoria V Gijón Juan J Navarro-Mari José María JM García-Salcedo José Antonio JA Soriano Miguel M
Scientific reports 20200812 1
Next generation sequencing methods are widely used in evaluating the structure and functioning of microbial communities, especially those centered on 16S rRNA subunit. Since Illumina Miseq, the most used sequencing platform, does not allow the full sequencing of 16S rRNA gene, this study aims to evaluate whether the choice of different target regions might affect the outcome of microbiome studies regarding soil and saliva samples. V1V3, V3V4, V4V5 and V6V8 domains were studied, finding that whil ...[more]
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