Ontology highlight
ABSTRACT:
SUBMITTER: Lim D
PROVIDER: S-EPMC7426819 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Nature communications 20200813 1
Genetically fusing protein domains to Cas9 has yielded several transformative technologies; however, the genetic modifications are limited to natural polypeptide chains at the Cas9 termini, which excludes a diverse array of molecules useful for gene editing. Here, we report chemical modifications that allow site-specific and multiple-site conjugation of a wide assortment of molecules on both the termini and internal sites of Cas9, creating a platform for endowing Cas9 with diverse functions. Usi ...[more]