Ontology highlight
ABSTRACT:
SUBMITTER: Boroviak K
PROVIDER: S-EPMC4819711 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
Boroviak Katharina K Doe Brendan B Banerjee Ruby R Yang Fengtang F Bradley Allan A
Genesis (New York, N.Y. : 2000) 20160125 2
Deletions, duplications, and inversions of large genomic regions covering several genes are an important class of disease causing variants in humans. Modeling these structural variants in mice requires multistep processes in ES cells, which has limited their availability. Mutant mice containing small insertions, deletions, and single nucleotide polymorphisms can be reliably generated using CRISPR/Cas9 directly in mouse zygotes. Large structural variants can be generated using CRISPR/Cas9 in ES c ...[more]