Dataset Information

Complete Genome Sequence and Manual Reannotation of Mycobacterium avium subsp. paratuberculosis Strain DSM 44135.

ABSTRACT: Here, we report the complete genome sequence of the Mycobacterium avium subsp. paratuberculosis reference strain DSM 44135, amended with a manual genome reannotation. The strain was originally described as M. paratuberculosis strain 6783. It was isolated from feces from a dairy cow in northern Germany.

SUBMITTER: Goethe R

PROVIDER: S-EPMC7427190 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Complete Genome Sequence and Manual Reannotation of Mycobacterium avium subsp. paratuberculosis Strain DSM 44135.

Goethe Ralph R Basler Tina T Meissner Thorsten T Goethe Elke E Spröer Cathrin C Swiderski Jolantha J Gerlach Gerald-F GF Weiss Siegfried S Jarek Michael M Bunk Boyke B

Microbiology resource announcements 20200813 33

Here, we report the complete genome sequence of the Mycobacterium avium subsp. paratuberculosis reference strain DSM 44135, amended with a manual genome reannotation. The strain was originally described as M. paratuberculosis strain 6783. It was isolated from feces from a dairy cow in northern Germany. ...[more]

PMID: 32817152

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Project description:BackgroundTwo genotypically and microbiologically distinct strains of Mycobacterium avium subsp. paratuberculosis (MAP) exist - S and C MAP strains that primarily infect sheep and cattle, respectively. Concentration of iron in the cultivation medium has been suggested as one contributing factor for the observed microbiologic differences. We recently demonstrated that S strains have defective iron storage systems, leading us to propose that these strains might experience iron toxicity when excess iron is provided in the medium. To test this hypothesis, we carried out transcriptional and proteomic profiling of these MAP strains under iron-replete or -deplete conditions.ResultsWe first complemented M.smegmatis?ideR with IdeR of C MAP or that derived from S MAP and compared their transcription profiles using M. smegmatis mc(2)155 microarrays. In the presence of iron, sIdeR repressed expression of bfrA and MAP2073c, a ferritin domain containing protein suggesting that transcriptional control of iron storage may be defective in S strain. We next performed transcriptional and proteomic profiling of the two strain types of MAP under iron-deplete and -replete conditions. Under iron-replete conditions, C strain upregulated iron storage (BfrA), virulence associated (Esx-5 and antigen85 complex), and ribosomal proteins. In striking contrast, S strain downregulated these proteins under iron-replete conditions.. iTRAQ (isobaric tag for relative and absolute quantitation) based protein quantitation resulted in the identification of four unannotated proteins. Two of these were upregulated by a C MAP strain in response to iron supplementation. The iron-sparing response to iron limitation was unique to the C strain as evidenced by repression of non-essential iron utilization enzymes (aconitase and succinate dehydrogenase) and upregulation of proteins of essential function (iron transport, [Fe-S] cluster biogenesis and cell division).ConclusionsTaken together, our study revealed that C and S strains of MAP utilize divergent metabolic pathways to accommodate in vitro iron stress. The knowledge of the metabolic pathways these divergent responses play a role in are important to 1) advance our ability to culture the two different strains of MAP efficiently, 2) aid in diagnosis and control of Johne's disease, and 3) advance our understanding of MAP virulence.

Dataset Information

Complete Genome Sequence and Manual Reannotation of Mycobacterium avium subsp. paratuberculosis Strain DSM 44135.

Publications

Complete Genome Sequence and Manual Reannotation of Mycobacterium avium subsp. <i>paratuberculosis</i> Strain DSM 44135.

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