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Profiling of miRNAs in porcine Sertoli cells.

ABSTRACT: Background:Sertoli cells (SCs) create a specialized environment to support and dictate spermatogenesis. MicroRNAs (miRNAs), a kind of ~?22?nt small noncoding RNAs, have been reported to be highly abundant in mouse SCs and play critical roles in spermatogenesis. However, the miRNAs of porcine SCs remain largely unknown. Methods:We isolated porcine SCs and conducted small RNA sequencing. By comparing miRNAs in germ cells, we systematically analyzed the miRNA expression pattern of porcine SCs. We screened the highly enriched SC miRNAs and predicted their functions by Gene Ontology analysis. The dual luciferase assay was used to elucidate the regulation of tumor necrosis factor receptor (TNFR)-associated factor 3 (TRAF3) by ssc-miR-149. Results:The analysis showed that 18 miRNAs were highly expressed in SCs and 15 miRNAs were highly expressed in germ cells. These miRNAs were predicted to mediate SC and germ cell functions. In addition, ssc-miR-149 played critical roles in SCs by targeting TRAF3. Conclusion:Our findings provide novel insights into the miRNA expression pattern and their regulatory roles of porcine SCs.

SUBMITTER: Chen X

PROVIDER: S-EPMC7429792 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Profiling of miRNAs in porcine Sertoli cells.

Chen Xiaoxu X Zheng Yi Y Li Xueliang X Gao Qiang Q Feng Tongying T Zhang Pengfei P Liao Mingzhi M Tian Xiu'e X Lu Hongzhao H Zeng Wenxian W

Journal of animal science and biotechnology 20200817

<h4>Background</h4>Sertoli cells (SCs) create a specialized environment to support and dictate spermatogenesis. MicroRNAs (miRNAs), a kind of ~ 22 nt small noncoding RNAs, have been reported to be highly abundant in mouse SCs and play critical roles in spermatogenesis. However, the miRNAs of porcine SCs remain largely unknown.<h4>Methods</h4>We isolated porcine SCs and conducted small RNA sequencing. By comparing miRNAs in germ cells, we systematically analyzed the miRNA expression pattern of po ...[more]

PMID: 32821380

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Profiling of miRNAs in porcine Sertoli cells.

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Profiling of miRNAs in porcine Sertoli cells.

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