Ontology highlight
ABSTRACT:
SUBMITTER: Coelho MA
PROVIDER: S-EPMC7431537 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Coelho Matthew A MA De Braekeleer Etienne E Firth Mike M Bista Michal M Lukasiak Sebastian S Cuomo Maria Emanuela ME Taylor Benjamin J M BJM
Nature communications 20200817 1
Precise genome editing using CRISPR-Cas9 is a promising therapeutic avenue for genetic diseases, although off-target editing remains a significant safety concern. Guide RNAs shorter than 16 nucleotides in length effectively recruit Cas9 to complementary sites in the genome but do not permit Cas9 nuclease activity. Here we describe CRISPR Guide RNA Assisted Reduction of Damage (CRISPR GUARD) as a method for protecting off-targets sites by co-delivery of short guide RNAs directed against off-targe ...[more]