Dataset Information
High intensity exercise downregulates FTO mRNA expression during the early stages of recovery in young males and females.
Ontology highlight
ABSTRACT: Background
Physical exercise and activity status may modify the effect of the fat mass- and obesity-associated (FTO) genotype on body weight and obesity risk. To understand the interaction between FTO's effect and physical activity, the present study investigated the effects of high and low intensity exercise on FTO mRNA and protein expression, and potential modifiers of exercise-induced changes in FTO in healthy-weighted individuals.Methods
Twenty-eight untrained males and females (25.4?±?1.1?years; 73.1?±?2.0?kg; 178.8?±?1.4?cm; 39.0?±?1.2?ml.kg.min-?1 VO2peak) were genotyped for the FTO rs9939609 (T?>?A) polymorphism and performed isocaloric (400?kcal) cycle ergometer exercise on two separate occasions at different intensities: 80% (High Intensity (HI)) and 40% (Low Intensity (LO)) VO2peak. Skeletal muscle biopsies (vastus lateralis) and blood samples were taken pre-exercise and following 10 and 90 mins passive recovery.Results
FTO mRNA expression was significantly decreased after HI intensity exercise (p?=?0.003). No differences in basal and post-exercise FTO protein expression were evident between FTO genotypes. Phosphorylated adenosine monophosphate-activated protein kinase (AMPK) and Akt substrate of 160?kDa (AS160) were significantly increased following HI intensity exercise (p?p?>?0.05). However, skeletal muscle glucose accumulation at 10 mins following HI (p?=?0.021) and LO (p?=?0.033) intensity exercise was greater in AA genotypes compared to TT genotypes.Conclusion
Our novel data provides preliminary evidence regarding the effects of exercise on FTO expression in skeletal muscle. Specifically, high intensity exercise downregulates expression of FTO mRNA and suggests that in addition to nutritional regulation, FTO could also be regulated by exercise.Trial registration
ACTRN12612001230842. Registered 21 November 2012 - Prospectively registered, https://www.anzctr.org.au/.
SUBMITTER: Danaher J
PROVIDER: S-EPMC7433063 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Publications
High intensity exercise downregulates <i>FTO</i> mRNA expression during the early stages of recovery in young males and females.
Nutrition & metabolism 20200817
<h4>Background</h4>Physical exercise and activity status may modify the effect of the fat mass- and obesity-associated (<i>FTO</i>) genotype on body weight and obesity risk. To understand the interaction between FTO's effect and physical activity, the present study investigated the effects of high and low intensity exercise on FTO mRNA and protein expression, and potential modifiers of exercise-induced changes in FTO in healthy-weighted individuals.<h4>Methods</h4>Twenty-eight untrained males an ...[more]