Ontology highlight
ABSTRACT:
SUBMITTER: Chu N
PROVIDER: S-EPMC7438110 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Chu Nam N Viennet Thibault T Bae Hwan H Salguero Antonieta A Boeszoermenyi Andras A Arthanari Haribabu H Cole Philip A PA
eLife 20200803
Akt is a critical protein kinase that governs cancer cell growth and metabolism. Akt appears to be autoinhibited by an intramolecular interaction between its N-terminal pleckstrin homology (PH) domain and kinase domain, which is relieved by C-tail phosphorylation, but the precise molecular mechanisms remain elusive. Here, we use a combination of protein semisynthesis, NMR, and enzymological analysis to characterize structural features of the PH domain in its autoinhibited and activated states. W ...[more]