Ontology highlight
ABSTRACT:
SUBMITTER: Tang TMS
PROVIDER: S-EPMC7441500 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Tang T M Simon TMS Cardella Davide D Lander Alexander J AJ Li Xuefei X Escudero Jorge S JS Tsai Yu-Hsuan YH Luk Louis Y P LYP
Chemical science 20200512 23
Asparaginyl endopeptidases (AEPs) are ideal for peptide and protein labeling. However, because of the reaction reversibility, a large excess of labels or backbone modified substrates are needed. In turn, simple and cheap reagents can be used to label N-terminal cysteine, but its availability inherently limits the potential applications. Aiming to address these issues, we have created a chemo-enzymatic labeling system that exploits the substrate promiscuity of AEP with the facile chemical reactio ...[more]