Ontology highlight
ABSTRACT:
SUBMITTER: Fottner M
PROVIDER: S-EPMC9335880 | biostudies-literature | 2022 Jul
REPOSITORIES: biostudies-literature
Fottner Maximilian M Heimgärtner Johannes J Gantz Maximilian M Mühlhofer Rahel R Nast-Kolb Timon T Lang Kathrin K
Journal of the American Chemical Society 20220718 29
Asparaginyl endopeptidases (AEPs) have recently been widely utilized for peptide and protein modification. Labeling is however restricted to protein termini, severely limiting flexibility and scope in creating diverse conjugates as needed for therapeutic and diagnostic applications. Here, we use genetic code expansion to site-specifically modify target proteins with an isopeptide-linked glycylglycine moiety that serves as an acceptor nucleophile in AEP-mediated transpeptidation with various prob ...[more]