Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Cell Invasion through Integrin ?6 Upregulation in Colorectal Cancer.

ABSTRACT: The metastatic potential of colorectal cancer (CRC) is intensively promoted by the tumor microenvironment (TME) in a paracrine manner. As a pleiotropic inflammatory cytokine, Interleukin-6 (IL-6) is produced and involved in CRC, the same scenario where integrin ?v?6 also becomes upregulated. However, the relationship between IL-6 and integrin ?v?6 as well as their involvement in the crosstalk between CRC and TME remains largely unclear. In the present study, we demonstrated a positive correlation between the expression of IL-6 and integrin ?6 in CRC samples. The mutually promotive interaction between CRC and TME was further determined by an indirect coculture system. CRC cells could augment the secretion of IL-6 from fibroblasts, which in return induced invasion and integrin ?6 expression of CRC cells. Through the classic IL-6 receptor/STAT-3 signaling pathway, IL-6 mediated the upregulation of integrin ?6, which was involved in the invasion and epithelial-mesenchymal transition of CRC cells induced by IL-6. Taken together, our results reveal a paracrine crosstalk between IL-6 signals originating from the TME and increased the integrin ?6 level of CRC. IL-6 induces CRC invasion via upregulation of integrin ?6 through the IL-6 receptor/STAT-3 signaling pathway. Combined inhibition of IL-6 along with integrin ?6-targeted strategy may indicate new directions for antitumor strategies for CRC.

SUBMITTER: Sun Q 

PROVIDER: S-EPMC7443035 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature