Ontology highlight
ABSTRACT:
SUBMITTER: Tamura S
PROVIDER: S-EPMC7445056 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Tamura Saaki S Yasuoka Yukiko Y Miura Hiromi H Takahashi Gou G Sato Masahiro M Ohtsuka Masato M
Experimental animals 20200211 3
The pronuclear injection (PI)-based targeted transgenesis (PITT) method allows the generation of targeted transgenic (Tg) mice wherein a single copy of a transgene is integrated into the Rosa26 locus following PI. The Rosa26 locus allows unbiased ubiquitous expression of integrated transgenes; however, it remains little known whether tissue-specific promoters retain their functional properties when placed at the Rosa26 locus. We evaluated tissue-specific activity and reproducibility of exogenous ...[more]