Metabolic modeling of bacterial co-culture systems predicts enhanced carbon monoxide-to-butyrate conversion compared to monoculture systems.

ABSTRACT: We used metabolic modeling to computationally investigate the potential of bacterial coculture system designs for CO conversion to the platform chemical butyrate. By taking advantage of the native capabilities of wild-type strains, we developed two anaerobic coculture designs by combining Clostridium autoethanogenum for CO-to-acetate conversion with bacterial strains that offer high acetate-to-butyrate conversion capabilities: the environmental bacterium the human gut bacteriumEubacterium rectale. When grown in continuous stirred tank reactor on a 70/0/30 CO/H₂/N₂ gas mixture, the C. autoethanogenum-C Kluyveri co-culture was predicted to offer no mprovement in butyrate volumetric productivity compared to an engineered C. autoethanogenum mon

SUBMITTER: Li X 

PROVIDER: S-EPMC7449128 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature