Dataset Information

Developmental changes in myocardial B cells mirror changes in B cells associated with different organs.

ABSTRACT: The naive heart harbors a population of intravascular B cells that make close contact with the cardiac microvasculature. However, the timing of their appearance and their organ specificity remain unknown. To address this knowledge gap, we performed a systematic analysis of B cells isolated from the myocardium and other organs, from embryonic life to adulthood. We found that the phenotype of myocardial B cells changed dynamically during development. While neonatal heart B cells were mostly CD11b+ and CD11b- CD21-CD23-, adult B cells were predominantly CD11b-CD21+CD23+. Histological analysis and intravital microscopy of lung and liver showed that organ-associated B cells in contact with the microvascular endothelium were not specific to the heart. Flow cytometric analysis of perfused hearts, livers, lungs, and spleen showed that the dynamic changes in B cell subpopulations observed in the heart during development mirrored changes observed in the other organs. Single cell RNA sequencing (scRNAseq) analysis of B cells showed that myocardial B cells were part of a larger population of organ-associated B cells that had a distinct transcriptional profile. These findings broaden our understanding of the biology of myocardial-associated B cells and suggest that current models of the dynamics of naive B cells during development are incomplete.

SUBMITTER: Rocha-Resende C

PROVIDER: S-EPMC7455131 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Developmental changes in myocardial B cells mirror changes in B cells associated with different organs.

Rocha-Resende Cibele C Yang Wei W Li Wenjun W Kreisel Daniel D Adamo Luigi L Mann Douglas L DL

JCI insight 20200820 16

The naive heart harbors a population of intravascular B cells that make close contact with the cardiac microvasculature. However, the timing of their appearance and their organ specificity remain unknown. To address this knowledge gap, we performed a systematic analysis of B cells isolated from the myocardium and other organs, from embryonic life to adulthood. We found that the phenotype of myocardial B cells changed dynamically during development. While neonatal heart B cells were mostly CD11b+ ...[more]

PMID: 32663200

Similar Datasets

Project description:Phelipanche aegyptiaca Pers. is a root holoparasitic plant considered to be among the most destructive agricultural weeds worldwide. In order to gain more knowledge about the metabolic profile of the parasite during its developmental stages, we carried out primary metabolic and lipid profiling using GC-MS analysis. In addition, the levels of amino acids that incorporate into proteins, total protein in the albumin fraction, nitrogen, reduced sugars, and phenols were determined. For the assays, the whole plants from the four developmental stages-tubercle, pre-emergent shoot, post-emergent shoot, and mature flowering plants-were taken. Thirty-five metabolites out of 66 differed significantly between the various developmental stages. The results have shown that the first three developmental stages were distinguished in their profiles, but the latter two did not differ from the mature stage. Yet, 46% of the metabolites detected did not change significantly during the developmental stages. This is unlike other studies of non-parasitic plants showing that their metabolic levels tend to alter significantly during development. This implies that the parasite can control the levels of these metabolites. We further studied the metabolic nature of five organs (adventitious roots, lower and upper shoot, floral buds, and flowers) in mature plants. Similar to non-parasitic plants, the parasite exhibited significant differences between the vegetative and reproductive organs. Compared to other organs, floral buds had higher levels of free amino acids and total nitrogen, whereas flowers accumulated higher levels of simple sugars such as sucrose, and the putative precursors for nectar synthesis, color, and volatiles. This suggests that the reproductive organs have the ability to accumulate metabolites that are required for the production of seeds and as a source of energy for the reproductive processes. The data contribute to our knowledge about the metabolic behavior of parasites that rely on their host for its basic nutrients.

Dataset Information

Developmental changes in myocardial B cells mirror changes in B cells associated with different organs.

Publications

Developmental changes in myocardial B cells mirror changes in B cells associated with different organs.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets