Dataset Information

Interferon gamma, TGF-?1 and RANTES expression in upper airway samples from SARS-CoV-2 infected patients.

ABSTRACT: Upper respiratory tract is the primary site of SARS-CoV-2 replication. Releasing of pro and anti-inflammatory mediators plays an important role in the immunopathogenesis of Coronavirus Disease 2019 (COVID-19). The aim of this study was to evaluate the early inflammatory response in upper airway by measuring of IFN-?, TGF-?1 and RANTES at mRNA level. Forty five SARS-CoV-2 infected patients were enrolled, whose were divided in two groups: asymptomatic and symptomatic. Twenty healthy persons, SARS-CoV-2 negative were included as controls. Higher IFN-? expression was detected in SARS-CoV-2 infected patients in comparison with controls (p = 0.0393). IFN-? expression was increased in symptomatic patients (p = 0.0405). TGF-?1 and RANTES expressions were lower in SARS-CoV-2 infected patients than controls (p < 0.0001; p = 0.0011, respectively). A significant correlation between IFN-? and TGF-?1 was observed in SARS-CoV-2 asymptomatic patients (r = +0.61, p = 0.0014). The findings suggest that imbalance between IFN-? and TGF-?1 expression could be an impact in clinical expression of SARS-CoV-2 infection.

SUBMITTER: Montalvo Villalba MC

PROVIDER: S-EPMC7455570 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Interferon gamma, TGF-β1 and RANTES expression in upper airway samples from SARS-CoV-2 infected patients.

Montalvo Villalba María Caridad MC Valdés Ramírez Odalys O Muné Jiménez Mayra M Arencibia Garcia Amely A Martinez Alfonso Javier J González Baéz Guelsy G Roque Arrieta Rosmery R Rosell Simón Dianelvys D Alvárez Gainza Delmis D Sierra Vázquez Beatriz B Resik Aguirre Sonia S Guzmán Tirado Maria Guadalupe MG

Clinical immunology (Orlando, Fla.) 20200829

Upper respiratory tract is the primary site of SARS-CoV-2 replication. Releasing of pro and anti-inflammatory mediators plays an important role in the immunopathogenesis of Coronavirus Disease 2019 (COVID-19). The aim of this study was to evaluate the early inflammatory response in upper airway by measuring of IFN-γ, TGF-β1 and RANTES at mRNA level. Forty five SARS-CoV-2 infected patients were enrolled, whose were divided in two groups: asymptomatic and symptomatic. Twenty healthy persons, SARS- ...[more]

PMID: 32866645

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Project description:SARS-CoV-2 infection causing the COVID-19 pandemic calls for immediate interventions to avoid viral transmission, disease progression, and subsequent excessive inflammation and tissue destruction. Primary normal human bronchial epithelial cells are among the first targets of SARS-CoV-2 infection. Here, we show that ColdZyme medical device mouth spray efficiently protected against virus entry, excessive inflammation, and tissue damage. Applying ColdZyme to fully differentiated, polarized human epithelium cultured at an air-liquid interphase (ALI) completely blocked binding of SARS-CoV-2 and increased local complement activation mediated by the virus as well as productive infection of the tissue model. While SARS-CoV-2 infection resulted in exaggerated intracellular complement activation immediately following infection and a drop in transepithelial resistance, these parameters were bypassed by single pretreatment of the tissues with ColdZyme mouth spray. Crucially, our study highlights the importance of testing already evaluated and safe drugs such as ColdZyme mouth spray for maintaining epithelial integrity and hindering SARS-CoV-2 entry within standardized three-dimensional (3D) in vitro models mimicking the in vivo human airway epithelium.IMPORTANCE Although our understanding of COVID-19 continuously progresses, essential questions regarding prophylaxis and treatment remain open. A hallmark of severe SARS-CoV-2 infection is a hitherto-undescribed mechanism leading to excessive inflammation and tissue destruction associated with enhanced pathogenicity and mortality. To tackle the problem at the source, transfer of SARS-CoV-2, subsequent binding, infection, and inflammatory responses have to be avoided. In this study, we used fully differentiated, mucus-producing, and ciliated human airway epithelial cultures to test the efficacy of ColdZyme medical device mouth spray in terms of protection from SARS-CoV-2 infection. Importantly, we found that pretreatment of the in vitro airway cultures using ColdZyme mouth spray resulted in significantly shielding the epithelial integrity, hindering virus binding and infection, and blocking excessive intrinsic complement activation within the airway cultures. Our in vitro data suggest that ColdZyme mouth spray may have an impact in prevention of COVID-19.

Dataset Information

Interferon gamma, TGF-?1 and RANTES expression in upper airway samples from SARS-CoV-2 infected patients.

Publications

Interferon gamma, TGF-β1 and RANTES expression in upper airway samples from SARS-CoV-2 infected patients.

Similar Datasets

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