Optimized polymer-based glucose release in microtiter plates for small-scale E. coli fed-batch cultivations.

ABSTRACT:

Background

Small-scale cultivation vessels, which allow fed-batch operation mode, become more and more important for fast and reliable early process development. Recently, the polymer-based feeding system was introduced to allow fed-batch conditions in microtiter plates. Maximum glucose release rates of 0.35 mg/h per well (48-well-plate) at 37 °C can be achieved with these plates, depending on the media properties. The fed-batch cultivation of fluorescent protein-expressing E. coli at oxygen transfer rate levels of 5 mmol/L/h proved to be superior compared to simple batch cultivations. However, literature suggests that higher glucose release rates than achieved with the currently available fed-batch microtiter plate are beneficial, especially for fast-growing microorganisms.

SUBMITTER: Keil T 

PROVIDER: S-EPMC7457294 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature