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Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018.


ABSTRACT: Human cytomegalovirus (HCMV) infections remain a neglected public health issue. The aim of the present study was to evaluate the frequency of HCMV congenital infections in newborns up to 1 month in the Sao Paulo State, from 2010 to 2018. The molecular characterization of HCMV-positive samples was also undertaken. Urine samples from 275 potential congenital HCMV-infected patients were tested by real-time Polymerase Chain Reaction (qPCR). HCMV-positive samples were amplified by conventional PCR targeting the UL89 gene, sequenced and searched for mutations. A total of 32 (11.6%) positive-HCMV cases were detected (mean Ct 30.59); mean and median age of 10.3 and 6 days old, respectively. Children aged between 0-3 weeks had higher HCMV detection rates (84.4%; 27/32). UL89 gene was successfully sequenced in two samples, both classified as the human betaherpesvirus 5. No described resistance-associated mutations were identified. A routine screening in newborns coupled with the genetic characterization of key viral genes is vital to decrease sequels associated with congenital HCMV infections.

SUBMITTER: Figueiredo CG 

PROVIDER: S-EPMC7458071 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Frequency of congenital cytomegalovirus infections in newborns in the Sao Paulo State, 2010-2018.

Figueiredo Carla Grasso CG   Luchs Adriana A   Durigon Edison Luiz EL   Oliveira Danielle Bruna Leal de DBL   Silva Vanessa Barbosa da VBD   Mello Ralyria Melyria RM   Afonso Ana Maria Sardinha AMS   Oliveira Maria Isabel de MI  

Revista do Instituto de Medicina Tropical de Sao Paulo 20200803


Human cytomegalovirus (HCMV) infections remain a neglected public health issue. The aim of the present study was to evaluate the frequency of HCMV congenital infections in newborns up to 1 month in the Sao Paulo State, from 2010 to 2018. The molecular characterization of HCMV-positive samples was also undertaken. Urine samples from 275 potential congenital HCMV-infected patients were tested by real-time Polymerase Chain Reaction (qPCR). HCMV-positive samples were amplified by conventional PCR ta  ...[more]

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