Tracking leukemic T-cell transcriptional dynamics in vivo with a blood-based reporter assay.
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ABSTRACT:
SUBMITTER: Tamayo AG
PROVIDER: S-EPMC7459418 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Publications
Tracking leukemic T-cell transcriptional dynamics in vivo with a blood-based reporter assay.
Tamayo Alfred G AG Shukor Syukri S Burr Alexandra A Erickson Patrick P Parekkadan Biju B
FEBS open bio 20200812 9
Transcriptional dynamics of cancer cells govern cell fate decisions and are therapeutically actionable drug targets. In this study, we engineered a circulating cancer cell line that secretes a luciferase reporter to capture constitutive and circadian clock-driven transcription dynamics over the course of a day. Engineered human leukemic T cells (Jurkat) were observed to rhythmically secrete luciferase in a continuous flow cell culture system. When transplanted in vivo, engineered leukemic cells ...[more]
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